Original Research Article
Year: 2022 | Month: August | Volume: 9 | Issue: 8 | Pages: 1-6
DOI: https://doi.org/10.52403/ijrr.20220801
Prevalence and Antibiotic Susceptibility Pattern of Staphylococcus Aureus Isolated from Blood Samples
Mahesh Chandra1, Amandeep Kaur2
1M. Sc. Medical Microbiology student, Department of Microbiology, Adesh Institute of Medical Science and Research, Bathinda.
2Professor, Department of Microbiology, Adesh Institute of Medical Science and Research, Bathinda.
Corresponding Author: Amandeep Kaur
ABSTRACT
Introduction: Staphylococcus aureus (S. aureus) is a Gram-positive cocci arranged in grape like clusters, non-motile, non-sporing, non-capsulated, that causes nosocomial infections, severe blood infections, bacteremia, food poisoning, cutaneous infections, toxic shock syndrome.
Objectives: To isolate and identify S. aureus from blood samples.
To perform antimicrobial susceptibility testing of S. aureus.
To find prevalence of bacteremia caused due to S. aureus.
Material & Methods: The study was carried out from October 2021 to March 2022, A total 1,270 samples were received in Bacteriology section from patients admitted in various ICUs & EMR of AIMSR hospital. S. aureus was identified on the basis of colony characteristics, gram staining morphology and biochemical tests. Antimicrobial susceptibility testing was done by automated method, Vitek 2 compact machine using P628AST card.
Results: The prevalence of S. aureus in culture positive blood samples was 2.83%. Maximum S. aureus were obtained from CCU department and minimum from NICU & PICU. S. aureus isolation was slightly more in males 25(69.4%) than females 11 (30.6%) and was found to be same in the age group of 21-40 & 41-60 years (30.60%) as compared to other age groups. S. aureus isolates were highly sensitive to teicoplanin, vancomycin, tigecycline, doxycycline, minocycline (100%). Sensitivity towards linezolid was recorded as (96%), Tetracycline (86.1%) and Daptomycin (77.7%). However, they showed resistance to Benzylpenicillin, & Oxacillin (100%), Ciprofloxacin & Levofloxacin (83.3%), Erythromycin (69.5%), Cotrimoxazole (52.8%), Clindamycin (41.7%), Gentamicin (38.9%), Daptomycin (22.3%), Tetracycline(13.9%), Rifampicin (5.6%), Linezolid (3%).
Conclusion: S. aureus bacteremia causes a health burden, particularly in low and middle income countries.
S. aureus infections are a significant clinical problem in medical practice as the organism shows resistance to the commonly used first line drugs.
Keywords: Staphylococcus aureus, Gram positive cocci, Bacteremia, methicillin resistant S. aureus, antimicrobial susceptibility testing.
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