Original Research Article
Year: 2020 | Month: July | Volume: 7 | Issue: 7 | Pages: 412-416
Study of Aerobic Bacterial Isolates and Their Antibiogram from Pus Sample in Government General Hospital, Guntur
B. Nirmala Grace, K. Ravi Kiran, B. Venkata Rao
Department of Microbiology, Guntur Medical College, Guntur.
Corresponding Author: K. Ravi Kiran
ABSTRACT
Introduction: Pyogenic wound infection is a major cause of morbidity because of emergence of multidrug resistant bacterial strains. Hence it is important to know the antibiotic sensitivity pattern of the isolates from pus samples to choose right antibiotics for the management of patient.
Aim: To isolate the aerobic bacteria from pus samples and study their antibiotic sensitivity pattern.
Methods: 524 samples received in the Department of Microbiology, Guntur medical college were processed as per the standard guidelines in the laboratory .All the bacterial isolates obtained were tested for antibiotic sensitivity by Kirby Bauer disc diffusion method and interpretation was done as per CLSI guide lines.
Results: Out of 524 samples tested 476 (90.8%) were culture positive and 48 (9.2%) were culture sterile. Out of 476 culture positive samples 73.7% were Gram negative bacilli and 26.3% were Gram positive cocci. Majority of GNB were Klebsiella pneumoniae (29.5%) followed by Pseudomonas (23.3%), Escherichia coli (14.4%) and Proteus (3.5%). Staphylococcus aureus (22%) and Coagulase negative staphylococci (3.8%) were isolated. Pure cultures were obtained in 88.2% and mixed cultures in 11.8%. Most of the GNB were sensitive to aminoglycosides, carbapenems, Piperacillin Tazobactam, third generation cephalosporins and fluoroquinolones. MRSA were isolated in 27% of Staphylococcus aureus strains.
Conclusion: Empirical and appropriate use of antibiotics is very crucial in preventing emergence of multidrug resistant bacteria. Hence there is a need for such studies to know the current status of bacterial etiology and their antibiogram.
Keywords: Pus, Antibiogram, Aerobic bacteria, Klebsiella spp, Pseudomonas, MRSA
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