Original Research Article
Year: 2020 | Month: October | Volume: 7 | Issue: 10 | Pages: 76-94
Phenotypic Characterization of Salmonella Enterica from Chickens in Some Selected Local Governments of Yobe State
Ahmed, Idriss Jajere1, Mailafia, Samuel2, Abubakar, B. Mustapha3, Raji, A.Mashood4
1Graduate Assistant, Dept of Biological sciences, Federal University Gashua, Yobe state, Nigeria.
2Professor, Dept of Veterinary Microbiology, University of Abuja, Nigeria.
3Professor, Dept of Veterinary Microbiology, University of Maiduguri, Nigeria.
4Professor, Dept of Veterinary Microbiology, University of Ilorin, Nigeria.
Corresponding Author: Ahmed, Idriss Jajere
ABSTRACT
Salmonella of poultry are zoonotic, microorganisms transmitted to humans via contact with infected poultry feces, meat, eggs and handling of young chickens particularly by children, or anything in the area where they live. This study was conducted to determine phenotypic characters of Salmonella enterica from chickens presented for slaughter in some selected LGA of Yobe State, Nigeria. A cloacal swab and blood samples were collected and transported on ice pack to Veterinary Microbiology Laboratory, University of Maiduguri and analyzed for the presence of Salmonella enterica. Samples were then plate on Xylose Lysine Deoxycholate agar showing blackish colonies. A total of 600 (300cloacal swab and 300 blood), consisting (202 males, 98 female chickens, 150 local and exotic each) were randomly sampled in 16 weeks for the isolation of Salmonella enterica. The result show that72 (24.00%) S. enterica were isolated from cloacal swab where 8 (2.66%) from blood samples while, 24 (24.48%) from female chickens, 66 (32.67%) from male chickens and 64 (42.66%) from local chickens, Similarly 16 (10.66%) from exotic chickens and. The overall isolation rate was 80 (13.33%).All the 80 presumptive positive isolates showed positive for Catalase, Citrate, H2S, Mortility test, Methyl red and Tripple sugar ion tests. While negative for Gram stain, Oxidase, VP, Indole and Urease tests. The presumptive Salmonella isolates were subjected to MicrobactTM GNB 24E System kit, where 40 selected presumptive isolates (36 from blood and 4 from cloacal swab) tested using Microbact 24E GNB Computerize system 10 (9 (45%) from cloacal swab and 1 (5%) from blood) samples were found to be positive for Salmonella organism. All the blood samples were tested for agglutination using slide method, 255 were found to be positive, showing a wide range with positives after blood culture 8. It is therefore, concluded that Salmonella organisms are prevalent in some selected local governments of Yobe State. It is therefore, recommended that more research should be conducted on other poultry such as ducks and goose which could reservoir of salmonella organism in the study area.
Keywords: Salmonella, Phenotypic, Characterization, Chickens.
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