Original Research Article
Year: 2019 | Month: December | Volume: 6 | Issue: 12 | Pages: 352-359
Comparative Research Study of in vitro Antioxidant Activity of Leaves Calli and Diverse Geographical Leaves Samples From Ethnomedicinal Herb Achyranthes Aspera L
Aziz Ahmed1, Mrunal K. Shirsat2, Mohd Shuaib3, Rizwan Ahmad4, Ankita Sharma5
1Research Scholar, 2Professor and Researcher,
Pacific Academy of Higher Education and Research University, Udaipur, Rajasthan, India, 313003,
3Professor and Researcher at Department of Pharmacy, Kalka Institute for Research & Advanced Studies, Meerut, U.P, India, 250002,
4Department of Biotechnology, Mewar University, Chittorgarh, Rajasthan, India, 312901,
5Department of Pharmacy, Mewar University, Chittorgarh, Rajasthan, India, 312901,
Corresponding Author: Aziz Ahmed
ABSTRACT
Background: Achyranthes aspera is an ethnomedicinal herb belongs to family Amaranthaceae. It contains alkaloids, flavonoids, tannins, anthraquinones, saponins, glycosides and unstable oils. The plant is extremely valued by traditional traders and utilized in cure of bronchospasm, internal bleeding, uterine tonic, respiratory infections, cold, chronic wheeze, pain in stomach, weakness, dysentery, ear troubles, pain in head, leucoderma, pneumonia, renal complications, scorpion bite, snake bite and skin diseases.
Aim: Determination of antioxidant activity was performed in leaves calli of and eight different geographical leaves samples of A. aspera L.
Material and method: Leaves calli with different concentrations and combination of auxins and cytokinins like 2, 4-Dichlorophenoxyacetic acid (2,4-D), Indole Acetic Acid (IAA), Naphthalene Acetic Acid (NAA) and 6-Benzyl Amino Purine (BAP) were maintained. Three methods DPPH radical scavenging activity, H2O2 scavenging activity and ferric reducing power were applied for determination of antioxidant activities of leaves calli and leaves samples.
Result Highest DPPH radical scavenging activity (85.31%), H2O2 scavenging activity (75.31%) and ferric reducing power (25.3µg/g) were observed in calli supplemented with 2,4-D (2.0 mg/l) + IAA (0.5mg/l).
Conclusion: Excellent in vitro antioxidant activity was given by extract of callus culture on MS medium supplemented with 2,4-D (2.0 mg/l) + IAA (0.5mg/l). So they can be used as natural antioxidants after isolation and purification.
Key words: MS medium, Antioxidant activity, Leaves calli, Auxin , Cytokinin
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