Year: 2026 | Month: May | Volume: 13 | Issue: 5 | Pages: 116-120
DOI: https://doi.org/10.52403/ijrr.20260509
Characteristics of Hydrolyzed Snakehead (Channa striata) Fish Protein Using Pepsin Enzyme
Siti Aisyah1, Iin Khusnul Khotimah1, Hafni Rahmawati1, Purnomo1, Agustiana1
1Department of Fish Product Technology, Fisheries and Marine Science Faculty, Lambung Mangkurat University, Banjarbaru, Indonesia.
Corresponding Author: Hafni Rahmawati
ABSTRACT
Snakehead fish (Channa striata) is a high-value fishery commodity with high protein content, especially albumin. However, optimal utilization remains a challenge, especially for the meat, which has so far only been sold whole or as fillets. Meeting the demand for fish protein hydrolysate is highly beneficial as a source of amino acids, functional food ingredients, and dietary supplements, as well as for increasing the added value of snakehead fish into a superior product in the form of HPI using pepsin enzymes. This study aims to obtain the best results from variations in concentration and hydrolysis time on the functional properties of HPI. This study will evaluate the effect of enzyme concentration variations (1 and 2%) and hydrolysis time (4 and 6 hours) on yield, degree of hydrolysis, and protein content. The method used is a laboratory experiment with a completely randomized factorial design. Data analysis showed that yield was not affected by concentration or time factors or the interaction between the two, while the degree of hydrolysis was significantly affected by concentration and the interaction between the two factors but was significantly not affected by time. In contrast, protein content was significantly affected by time but was significantly not affected by concentration and the interaction between concentration and time. The best results based on yield, degree of hydrolysis, and protein content of snakehead fish HPI were obtained using a pepsin enzyme concentration of 2% and a hydrolysis time of 6 hours.
Keywords: amino acids, degree of hydrolysis, molecular weight, proximate analysis, yield
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