Year: 2024 | Month: February | Volume: 11 | Issue: 2 | Pages: 548-555
DOI: https://doi.org/10.52403/ijrr.20240256
Cryptosporidiosis Detection Using Immunoglobulin a Serum with Enzyme-Linked Immunosorbent Assay Method
Deany Puteri Naila1, Nuzulia Irawati2, Malinda Meinapuri3, Hasmiwati2, Rikarni4
1Ungraduated Student of Faculty of Medicine, Andalas University, Padang, Indonesia
2Department of Parasitology, Faculty of Medicine, Andalas University, Padang, Indonesia,
3Department of Histology, Faculty of Medicine, Andalas University, Padang, Indonesia
4Department of Clinical Pathology and Laboratory Medicine, Faculty of Medicine, Andalas University, Padang, Indonesia
Corresponding Author: Nuzulia Irawati
ABSTRACT
Background: Cryptosporidiosis is still one of the most common intestinal protozoan infections found in developing countries, affecting children and adults. Cryptosporidium sp. infection activates the immune response by secreting high levels of specific Cryptosporidium IgA antibodies during the infection process. IgA can be examined by ELISA to establish a diagnosis of cryptosporidiosis.
Methods: The purpose of this study was to determine the results of Cryptosporidiosis detection with serum IgA ELISA. This study was a quantitative descriptive study with 39 samples collected from children to adults using consecutive sampling technique. Data obtained from the study results were processed by univariate analysis and presented in tabular form to see the frequency distribution.
Result: The results of the study involving 39 respondents revealed that the most prevalent age groups were 6-11 years old and 36-45 years old, with the majority being female. Among the 39 samples examined using the ELISA method, the Optical Density ranged from 0.059 to 1.393. One sample (2.56%) tested positive for IgA Cryptosporidium sp., specifically from age 55 years (16.6%) and male participants (20%).
Conclusion: The study concluded that the Optical Density value of the positive ELISA examination was 1.393 with one sample found positive for Cryptosporidiosis from a 55 years old male.
Keywords: [Cryptosporidium sp., IgA, ELISA]
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