Original Research Article
Year: 2023 | Month: July | Volume: 10 | Issue: 7 | Pages: 93-97
DOI: https://doi.org/10.52403/ijrr.20230715
Effect of Essential Oil of Basil Leaf (Ocimum basilicum) on Malondialdehyde (MDA) Levels in Wistar Rats with Excessive Physical Activity
Gede Agus Ari Tirtayasa1, I Made Jawi2, I Putu Adiartha Griadhi3, I Made Muliarta3, I Made Krisna Dinata3, I Gusti Ayu Artini2
1Biomedical Science Masters Study Program, Concentration of Basic Medical Science, Faculty of Medicine, Universitas Udayana, Bali, Indonesia
2Department of Pharmacology, Faculty of Medicine, Universitas Udayana, Bali, Indonesia
3Department of Physiology, Faculty of Medicine, Universitas Udayana, Bali, Indonesia
Corresponding Author: Gede Agus Ari Tirtayasa
ABSTRACT
Introduction: Excessive physical activity causes the formation of ROS and RNS in the body, which can damage cellular lipids, DNA and proteins. This is responsible for the formation of oxidants that cause the aging process. Malondialdehyde (MDA) is produced from lipid peroxidation and is the end product of oxidative stress. This study aims to prove that administration of basil leaf essential oil can reduce levels of MDA in Wistar rats with excessive physical activity.
Methods: This research was an experimental post-test only control group design using male rats as the object of research. The sample was divided into 2 groups, the treatment group which was given basil essential oil 30 minutes before physical activity, and the control group which was given 1 ml of aquabidest every day 30 minutes before excessive activity. Each group was given physical activity in the form of swimming for 60 minutes. Then on the 21st day, MDA levels were examined using the ELISA method on blood. Data is recorded and analyzed.
Results: The average (MDA) level in the control group was 3.229 nmol/mL (95% CI 2.823 – 3.635) with a standard deviation of 0.816. The lowest MDA level in the control group was 2.456 nmol/mL and the highest was 5.217 nmol/mL. The average MDA level in the treatment group was 2.356 nmol/mL (95% CI 2.204 – 2.508) with a standard deviation of 0.285 nmol/mL. The lowest MDA level in the treatment group was 1.768 nmol/mL and the highest was 2.772 nmol/mL.
Conclusion: Administration of basil essential oil resulted in lower MDA levels than controls in Wistar rats subjected to excessive physical activity.
Keywords: Malondialdehyde. Basil essential oil, physical activity
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