Original Research Article
Year: 2023 | Month: July | Volume: 10 | Issue: 7 | Pages: 1-6
DOI: https://doi.org/10.52403/ijrr.20230701
Application of Arabica Coffee (Coffea Arabica) Leaf Ethanol Extract Gel Reduces Expression of Matrix Metalloproteinase-1 and Increases the Amount of Collagen in Male Wistar Rats Exposed to Ultraviolet B Light
Dewa Ayu Agung Diah Hadiningrat1, Agung Wiwiek Indrayani2, Ketut Kwartantaya Winaya3, Luh Putu Iin Indrayani Maker4,
Ida Ayu Ika Wahyuniari5
Desak Ketut Ernawati2
1Master Program in Biomedical Science, Anti-Aging Medicine, Faculty of Medicine,Universitas Udayana, Bali, Indonesia
2Department of Pharmacology and Therapy, Faculty of Medicine, Universitas Udayana, Bali, Indonesia
3Department of Dermatovenereology, Faculty of Medicine, Universitas Udayana, Bali, Indonesia
4Department of Anatomical Pathology, Faculty of Medicine, Universitas Udayana, Bali, Indonesia
5Department of Histology, Faculty of Medicine, Universitas Udayana, Bali, Indonesia.
Corresponding Author: Dewa Ayu Agung Diah Hadiningrat
ABSTRACT
Introduction: This study aims to determine the effect of arabica coffee leaf ethanol extract gel on decreasing MMP-1 expression and increasing the amount of collagen in the skin of male Wistar rats (Strains) exposed to UV-B light.
Methods: This is a post-test only control group design experimental study using male rats as subjects. Each group was exposed to UV light and smeared with 5% coffee leaf ethanol extract cream every day. Then the back skin tissue was taken and the amount of collagen was examined by Picro Sirius Red staining and MMP1 levels were examined by ELISA staining.
Results: The mean MMP-1 levels in groups N, K, P1, P2, and P3 were 1.023±0.018 ng/ml, 1.578±0.28 ng/ml, 1.001±0.027 ng/ml, 0.775±0.042 ng/ml, and 0.595±0.076 ng/ml. The average percentage of collagen in groups N, K, P1, P2, and P3 was 75.63 ± 2.92%, 66.43 ± 1.67%, 72.83 ± 3.13%, 76.02 ± 3 .78, and 80.43 ± 2.12%. MMP-1 levels decreased with increasing doses of arabica coffee leaf extract gel. The mean percentage of collagen increased with increasing doses of arabica coffee leaf extract gel in experimental animals.
Conclusion: Arabica coffee leaf ethanol extract gel inhibited the increase in MMP-1 levels in male rats exposed to UV-B light. Administration of arabica coffee leaf ethanol extract gel can increase the amount of collagen in male rats exposed to UV-B light.
Keywords: Arabica coffee, metalloprtoeinase-1, collagen, UVB.
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