Research Paper
Year: 2023 | Month: May | Volume: 10 | Issue: 5 | Pages: 523-527
DOI: https://doi.org/10.52403/ijrr.20230560
Pathological Studies and Molecular Detection of Salmonellosis in Desi Chickens of Nagpur Region
Prashant M. Sonkusale1, Shubhangi R. Warke2, Chaitanya S1, Sukirti Sharma1
1Department of Veterinary Pathology
2Department of Veterinary Microbiology & Animal Biotechnology T & R Cell, Nagpur Veterinary College,
(MAFSU), Nagpur
Corresponding Author: Dr. Prashant M. Sonkusale
ABSTRACT
Avian salmonellosis is an important disease causing serious impediment to the development of poultry industry especially in developing countries. During present study, mortality due to Salmonellosis in six desi chicken flocks belonging to Nagpur region of Maharashtra was noticed. Characteristic lesions of necrotic foci on liver, omphalitis and catarrhal enteritis were noticed in Salmonella spp. affected chickens. Colourless colonies on MLA, light pink colonies on BGA and jet black colonies on XLD agar confirmed the Salmonella spp. infection. Clinical samples of liver collected from six desi chicken flocks were further confirmed as Salmonella spp. by PCR amplification of 423bp of InvA gene. The lesions, cultural characteristics along with PCR amplification of InvA gene confirmed Salmonella spp. infection in desi chickens of Nagpur region.
Keywords: Salmonellosis, desi chickens, pathology, molecular diagnosis
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